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rac1 generation fluorescence resonance energy transfer fret biosensor  (Addgene inc)


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    Structured Review

    Addgene inc rac1 generation fluorescence resonance energy transfer fret biosensor
    CD93 signaling activates <t>Rac1</t> at the cell edge of spreading cells. ( a ) <t>FRET</t> analysis on control (sh-unr) or CD93-silenced (sh-CD93) HUVECs transduced with a lentiviral construct expressing the Rac1 biosensor. Cells were fixed at the early phase of adhesion to the ECM. Representative confocal images of transduced cells before photobleaching (acceptor pre) are shown. Rectangles indicate the photobleached cell area. Magnifications of the photobleached area are shown (2.5×). The colored scale represents the color range of FRET efficiency. Scale bars, 20 µm. ( b ) Plot showing the fluorescence increase (% FRET efficiency) upon photobleaching at the cell edge of early spreading ECs ( n = 10 cells for sh-unr and n = 9 cells for sh-CD93). Data are presented as scatter plot. ** p < 0.01; Student t -test.
    Rac1 Generation Fluorescence Resonance Energy Transfer Fret Biosensor, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rac1+generation+fluorescence+resonance+energy+transfer+fret+biosensor/pmc08622518-66-22-6?v=Addgene+inc
    Average 93 stars, based on 10 article reviews
    rac1 generation fluorescence resonance energy transfer fret biosensor - by Bioz Stars, 2026-08
    93/100 stars

    Images

    1) Product Images from "CD93 Signaling via Rho Proteins Drives Cytoskeletal Remodeling in Spreading Endothelial Cells"

    Article Title: CD93 Signaling via Rho Proteins Drives Cytoskeletal Remodeling in Spreading Endothelial Cells

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms222212417

    CD93 signaling activates Rac1 at the cell edge of spreading cells. ( a ) FRET analysis on control (sh-unr) or CD93-silenced (sh-CD93) HUVECs transduced with a lentiviral construct expressing the Rac1 biosensor. Cells were fixed at the early phase of adhesion to the ECM. Representative confocal images of transduced cells before photobleaching (acceptor pre) are shown. Rectangles indicate the photobleached cell area. Magnifications of the photobleached area are shown (2.5×). The colored scale represents the color range of FRET efficiency. Scale bars, 20 µm. ( b ) Plot showing the fluorescence increase (% FRET efficiency) upon photobleaching at the cell edge of early spreading ECs ( n = 10 cells for sh-unr and n = 9 cells for sh-CD93). Data are presented as scatter plot. ** p < 0.01; Student t -test.
    Figure Legend Snippet: CD93 signaling activates Rac1 at the cell edge of spreading cells. ( a ) FRET analysis on control (sh-unr) or CD93-silenced (sh-CD93) HUVECs transduced with a lentiviral construct expressing the Rac1 biosensor. Cells were fixed at the early phase of adhesion to the ECM. Representative confocal images of transduced cells before photobleaching (acceptor pre) are shown. Rectangles indicate the photobleached cell area. Magnifications of the photobleached area are shown (2.5×). The colored scale represents the color range of FRET efficiency. Scale bars, 20 µm. ( b ) Plot showing the fluorescence increase (% FRET efficiency) upon photobleaching at the cell edge of early spreading ECs ( n = 10 cells for sh-unr and n = 9 cells for sh-CD93). Data are presented as scatter plot. ** p < 0.01; Student t -test.

    Techniques Used: Transduction, Construct, Expressing, Fluorescence



    Similar Products

    rac1 generation fluorescence resonance energy transfer fret biosensor  (Addgene inc)
    93
    Addgene inc rac1 generation fluorescence resonance energy transfer fret biosensor
    CD93 signaling activates <t>Rac1</t> at the cell edge of spreading cells. ( a ) <t>FRET</t> analysis on control (sh-unr) or CD93-silenced (sh-CD93) HUVECs transduced with a lentiviral construct expressing the Rac1 biosensor. Cells were fixed at the early phase of adhesion to the ECM. Representative confocal images of transduced cells before photobleaching (acceptor pre) are shown. Rectangles indicate the photobleached cell area. Magnifications of the photobleached area are shown (2.5×). The colored scale represents the color range of FRET efficiency. Scale bars, 20 µm. ( b ) Plot showing the fluorescence increase (% FRET efficiency) upon photobleaching at the cell edge of early spreading ECs ( n = 10 cells for sh-unr and n = 9 cells for sh-CD93). Data are presented as scatter plot. ** p < 0.01; Student t -test.
    Rac1 Generation Fluorescence Resonance Energy Transfer Fret Biosensor, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rac1+generation+fluorescence+resonance+energy+transfer+fret+biosensor/pmc08622518-66-22-6?v=Addgene+inc
    Average 93 stars, based on 1 article reviews
    rac1 generation fluorescence resonance energy transfer fret biosensor - by Bioz Stars, 2026-08
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    CD93 signaling activates Rac1 at the cell edge of spreading cells. ( a ) FRET analysis on control (sh-unr) or CD93-silenced (sh-CD93) HUVECs transduced with a lentiviral construct expressing the Rac1 biosensor. Cells were fixed at the early phase of adhesion to the ECM. Representative confocal images of transduced cells before photobleaching (acceptor pre) are shown. Rectangles indicate the photobleached cell area. Magnifications of the photobleached area are shown (2.5×). The colored scale represents the color range of FRET efficiency. Scale bars, 20 µm. ( b ) Plot showing the fluorescence increase (% FRET efficiency) upon photobleaching at the cell edge of early spreading ECs ( n = 10 cells for sh-unr and n = 9 cells for sh-CD93). Data are presented as scatter plot. ** p < 0.01; Student t -test.

    Journal: International Journal of Molecular Sciences

    Article Title: CD93 Signaling via Rho Proteins Drives Cytoskeletal Remodeling in Spreading Endothelial Cells

    doi: 10.3390/ijms222212417

    Figure Lengend Snippet: CD93 signaling activates Rac1 at the cell edge of spreading cells. ( a ) FRET analysis on control (sh-unr) or CD93-silenced (sh-CD93) HUVECs transduced with a lentiviral construct expressing the Rac1 biosensor. Cells were fixed at the early phase of adhesion to the ECM. Representative confocal images of transduced cells before photobleaching (acceptor pre) are shown. Rectangles indicate the photobleached cell area. Magnifications of the photobleached area are shown (2.5×). The colored scale represents the color range of FRET efficiency. Scale bars, 20 µm. ( b ) Plot showing the fluorescence increase (% FRET efficiency) upon photobleaching at the cell edge of early spreading ECs ( n = 10 cells for sh-unr and n = 9 cells for sh-CD93). Data are presented as scatter plot. ** p < 0.01; Student t -test.

    Article Snippet: The following plasmids were purchased from Addgene (Watertown, MA, USA): a lentiviral negative control vector containing scrambled shRNA (#1864, sh-unr) [ ]; Rac1 generation fluorescence resonance energy transfer (FRET) biosensor for lentivirus production (#66111, pLenti-Rac1-2G) [ ]; FRET-based biosensor reporting on Cdc42 activation (#68813, pLenti-Cdc42-2G) [ ]; RhoA second-generation FRET biosensor for lentivirus production (#40179, pLenti-RhoA-2G) [ ]. shRNA-mediated knockdown of CD93 was performed as previously outlined [ ] by using a pLKO.1 retroviral vector from the Mission shRNA Library (Merck KGaA, Darmstadt, Germany), which expresses a shRNA (clone TRCN0000029085) specific for the silencing of the human protein.

    Techniques: Transduction, Construct, Expressing, Fluorescence